Simple Western™ Technology provides automated capillary western analysis using capillary electrophoresis for protein separation followed by immunoassay-based detection within a fully enclosed system. Simple Western Technology simplifies and automates the traditional western blotting process by integrating it into a fully automated capillary system, providing more precise quantitative data than traditional western blotting due to its controlled and standardized operating conditions.

• Simple Western is a fully automated western technology. Traditional western blotting steps of sample separation, wash buffer incubations, and immunodetection of target proteins occur inside a hands-free instrument on your benchtop.
• Unlike traditional western blotting systems, Simple Western does not transfer proteins to a membrane, removing the inherent variability of western blotting and adding high-throughput sample processing and automated protein analysis.
• Tiny amounts of sample are separated inside capillaries and are cross linked to the capillary walls. Then, blocking and wash buffer incubations are performed using just a few microliters of your primary antibodies and conjugated secondary antibodies to detect target proteins in chemiluminescence and NIR/IR fluorescence channels (LOD <1 pg).