40% BioLyte 3/10 Ampholyte 10 mL 40% Solution – A Reliable Choice for High-Resolution Protein Separation
The 40% BioLyte 3/10 Ampholyte 10 mL 40% Solution stands out as a powerful tool designed to support high-resolution isoelectric focusing and other analytical applications. In modern biochemical research, precision is everything. Whether you’re working in protein characterization, electrophoresis, or Western blot workflows, using high-quality reagents directly impacts the accuracy of your results.
Engineered for dependable performance, this BioLyte solution provides a broad pH range (3–10), enabling effective separation of complex protein samples in both academic and industrial laboratories.
This article explores the features, benefits, and reasons why researchers choose this Ampholyte solution as a preferred lab reagent for electrophoresis and Western blot reagents workflows.
What is 40% BioLyte 3/10 Ampholyte Solution?
The 40% BioLyte 3/10 Ampholyte Solution is a high-quality amphoteric electrolyte mixture designed to create stable pH gradients during isoelectric focusing (IEF). With a concentration of 40%, this reagent ensures sharper protein band resolution and supports reproducible protein separation across the full pH spectrum of 3 to 10.
Its consistent performance and versatility make it a valuable lab chemical for molecular biology, proteomics, and analytical diagnostics.
Specifications of 40% BioLyte 3/10 Ampholyte 10 mL 40% solution
Code: 1631112
Catalog: Proteomics
Category: Ampholytes
Typology: 40%
Format: 10 ml
pH Range: 3.5–9.5
Temperature: RT
Standards: IEF

Key Features of 40% BioLyte 3/10 Ampholyte 10 mL 40% solution
- High-Concentration 40% Ampholyte Formula: Provides strong buffering capacity and supports precise pH gradient formation for enhanced separation.
- Wide pH Range (3–10): Suitable for resolving complex protein mixtures with diverse isoelectric points.
- Premium-Grade BioLyte Composition: Ensures stability, reproducibility, and purity in electrophoretic workflows.
- Ready-to-Use Solution: Supplied in a sterile 10 mL volume for convenient lab use and minimal preparation time.
- Compatible with Western Blot & Proteomics Workflows: Ideal for IEF, native gel systems, and sample preparation steps prior to blotting.
Benefits
- Superior Protein Resolution: The ampholyte formulation supports clear separation, enabling researchers to distinguish protein isoforms more effectively.
- Enhances Reproducibility: Stable pH gradients minimize variation between runs, improving data reliability in long-term studies.
- Versatile Laboratory Use: From proteomics to biochemical characterization, this reagent integrates smoothly into various electrophoresis and blot reagent workflows.
- Saves Time and Effort: Being a ready-made solution, it reduces prep time, allowing scientists to focus on analysis rather than reagent formulation.
- Trusted for High-Performance Western Blotting: Helps improve protein separation quality before blotting, leading to sharper and more accurate Western blot outcomes.
Applications
- Isoelectric focusing (IEF)
- Protein purification workflows
- 2D gel electrophoresis (as part of sample prep)
- Research involving protein charge profiling
- Pre-blotting optimization steps
- General electrophoresis requiring stable pH gradients
Conclusion
The 40% BioLyte 3/10 Ampholyte 10 mL 40% Solution is a dependable and efficient choice for researchers who require accuracy in protein separation and electrophoresis. Its high-purity formulation, wide pH range, and ready-to-use convenience make it a standout among modern lab reagents and Western blot reagents. With consistent performance and strong compatibility, this reagent supports improved resolution, reproducibility, and overall laboratory efficiency.
For scientists seeking a reliable lab chemical that delivers peak performance in proteomics and electrophoresis, this BioLyte Ampholyte solution is an essential addition to the laboratory.




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