Actinomycin D [Actinomycin C1] [Dactinomycin]: A Powerful Tool for Molecular Biology & Cell Research 

Actinomycin D, also known as Actinomycin C1 or Dactinomycin, is a potent antibiotic and transcription inhibitor widely used in molecular biology, cell culture, and biochemical research. As a high-value Lab Reagent, this compound plays a crucial role in studying gene expression, RNA synthesis, and cellular regulatory pathways. Researchers depend on Actinomycin D for its precision, reproducibility, and ability to selectively block transcriptional activity.

With applications ranging from apoptosis studies to RNA stability assays, Actinomycin D has become an essential reagent in advanced laboratories working with Lab Chemicals, Cell Culture Reagents, and Blot Reagents. 

What Is Actinomycin D [Actinomycin C1] [Dactinomycin]?

Actinomycin D is a Chromopeptide antibiotic derived from Streptomyces species. Its structure allows it to intercalate between DNA base pairs, effectively inhibiting RNA polymerase and blocking transcription. This powerful mechanism makes it a key research tool for:

• Gene expression studies 
• RNA half-life and mRNA stability analysis 
• Apoptosis and cell cycle research 
• Cancer biology models 
• DNA-binding studies 

Known also by its synonyms Actinomycin C1 and Dactinomycin, this reagent provides high accuracy in controlling transcriptional activity within experimental systems. 

Specifications of Actinomycin D [Actinomycin C1] [Dactinomycin]

Common Name: [Actinomycin C1] [Dactinomycin]
CAS Number: 50-76-0
Molecular Weight: 1255.5
Chemical Formula: C62H86N12O16
Solubility: DMSO, ethanol
Storage Temperature: 2-8°C
Appearance: Red orange solid
Identification: Pass
Purity: ≥ 98 %
Solubility: Soluble
BSE/TSE Free: BSE/TSE Free

Key Features of Actinomycin D [Actinomycin C1] [Dactinomycin]

• High-purity molecular biology grade: Ensures reliable performance in sensitive research assays.
• Strong transcription inhibitor: Effectively blocks RNA synthesis by binding to DNA.
• Versatile Lab Reagent: Widely used in cell culture, gene expression studies, and RNA analysis.
• Stable and consistent formulation: Designed for reproducible results across molecular biology workflows.
• Compatible with Blot Reagents and Cell Culture Reagents: Frequently used with Western blotting and protein studies when tracking gene regulation.
• Small effective concentrations: Highly potent, allowing cost-effective use in research labs. 

Benefits of Using Actinomycin D 

• Precise Control of Gene Expression: Actinomycin D enables researchers to halt transcription at specific time points, making it invaluable for studying transcription dynamics and regulatory events.
• Ideal for mRNA Stability & Decay Studies: Its inhibition of RNA synthesis helps assess RNA degradation rates and gene regulation mechanisms.
• Widely Used in Cancer Research: Because it disrupts DNA-dependent RNA synthesis, It is used extensively in tumor biology, drug screening, and apoptosis studies.
• Reliable for Molecular Biology Assays: Its consistent activity ensures accurate results in experiments involving RNA polymerase inhibition.
• Essential for Cell Culture Research: In cell culture systems, It is used to study cell signaling pathways, stress responses, and programmed cell death. 

Conclusion 

Actinomycin D (Actinomycin C1 / Dactinomycin) remains one of the most powerful transcription-inhibiting reagents available for molecular biology and cell culture research. Its ability to precisely block RNA synthesis makes it indispensable for gene expression studies, mRNA stability assays, and cancer research.

As a high-quality Lab Reagent, it offers the reliability, potency, and consistency required for advanced laboratory applications. Whether you’re working with Lab Chemicals, Blot Reagents, or Cell Culture Reagents, Actinomycin D is a trusted tool that supports accurate and reproducible scientific discovery.