Beta Mercaptoethanol 100 mL Reagent – A Critical Reducing Agent for Modern Molecular Biology 

The Beta Mercaptoethanol 100 mL Reagent is a premium-grade chemical designed to support sensitive laboratory workflows requiring accurate reduction, protein unfolding, and preservation. Beta Mercaptoethanol, also known as β-mercaptoethanol or 2-mercaptoethanol, is one of the most widely used reducing agents in biochemical, molecular biology, and protein-based research. Its powerful ability to break disulfide bonds makes it essential for sample preparation, denaturation, and stabilization of proteins. 

As a commonly used component of Western Blot Reagents, β-mercaptoethanol helps ensure proteins remain in their reduced, linearized form during SDS-PAGE and blotting applications. High-quality Lab Reagents such as this are essential for achieving clear, reproducible results in proteomics, molecular biology, and cell culture. 

Understanding Beta Mercaptoethanol (β-ME)

β-mercaptoethanol and 2-mercaptoethanol refer to the same chemical compound. It is widely recognized for its role in reducing disulfide bonds in proteins. In SDS-PAGE sample buffers, it helps ensure accurate protein separation based on molecular weight. 

Researchers often search for information such as beta mercaptoethanol molarity, molarity of β-mercaptoethanol, beta mercaptoethanol SDS, and β-mercaptoethanol storage because proper concentration and handling are critical for experimental success. 

Additionally, beta mercaptoethanol cell culture applications include maintaining reducing conditions for improved cell viability in specific media formulations. 

Specifications of Beta Mercaptoethanol 100 ml Reagent 

Origin: Germany, Spain, China
Alpha Vector: Mercaptoethanol
Melting Point: -100°C
Color: Yellow
Density: 1.110g/cm³
pH: 5.2
Boiling Point: 157°C
Packaging: Amber Glass/Poison Pack
Linear Formula: HSCH2CH2OH
Quantity: 100 mL
UN Number: 2966 

Chemical Specifications

CAS: 60-24-2
Molecular Formula: C2H6OS
Molecular Weight (g/mol): 78.13
MDL Number: MFCD00004890
InChI Key: DGVVWUTYPXICAM-UHFFFAOYSA-N
Synonym 2-mercaptoethanol, mercaptoethanol, thioglycol, beta-mercaptoethanol, ethanol, 2-mercapto, 2-thioethanol, 2-hydroxy-1-ethanethiol, thioethylene glycol, 2-hydroxyethanethiol, 2-hydroxyethyl mercaptanShow
PubChem CID: 1567
ChEBI CHEBI: 41218
IUPAC: Name 2-sulfanylethan-1-ol
SMILES: OCCS 

Key Features of Beta Mercaptoethanol 100 mL Reagent

• High-Purity Laboratory Grade: Ensures reliable performance in sensitive biochemical reactions.
• Essential Reducing Agent: Breaks disulfide bonds efficiently for accurate protein denaturation in SDS-PAGE. 
• Ideal for Western Blot Reagents & Protein Analysis: Ensures proteins remain in their reduced and unfolded state during electrophoresis.
• Consistent Composition & Performance: Suitable for reproducible results in proteomics and molecular biology experiments. 
• Convenient 100 mL Laboratory Pack: Easy to handle, measure, and store for frequent research use.
• Compatible With Various Blot Reagents: Works seamlessly with sample buffers, loading dyes, and gel systems. 

Benefits of Using Beta Mercaptoethanol 100 mL Reagent

• Ensures Accurate Protein Separation: By reducing disulfide bonds, β-mercaptoethanol guarantees proteins migrate correctly during SDS-PAGE.
• Enhances Reliability in Western Blotting: A vital part of Western Blot Reagents, it ensures proper protein unfolding for clearer, more accurate blot results.
• Supports Cell Culture Applications: Used at low concentrations to maintain reducing environments in specialized cell culture systems.
• High Stability and Controlled Reactivity: Proper β-mercaptoethanol storage helps maintain its reducing power and extends its usability.
• Widely Recognized in Biochemical Research: Used globally due to its effectiveness in protein denaturation and molecular analysis.
• Cost-Effective Laboratory Chemical: The 100 mL pack size is ideal for routine day-to-day laboratory use without unnecessary waste.
• Strong Reducing Properties With Multiple Uses: From β-mercaptoethanol SDS buffer preparation to maintaining protein integrity, it remains indispensable for researchers.

Conclusion

The Beta Mercaptoethanol 100 mL Reagent is a critical tool for protein-based research, biochemical analysis, electrophoresis, and Western blot applications. Its powerful reducing capabilities ensure proteins maintain their linear structure, enabling accurate size separation and reliable experimental data.

Whether you refer to it as beta mercaptoethanol, β-mercaptoethanol, or 2-mercaptoethanol, its role in maintaining reducing conditions is unmatched. As a high-quality Lab Reagent and essential item among Lab Chemicals and Blot Reagents, it continues to be a staple in modern research laboratories worldwide.