Beta Mercaptoethanol (50 mM in PBS): A Reliable Reducing Agent for Molecular Biology & Protein Research

Beta Mercaptoethanol (50 mM in PBS) is a widely used reagent in molecular biology, biochemistry, and protein analysis workflows. Prepared at a convenient working concentration, this ready-to-use formulation provides a powerful and consistent reducing environment for various laboratory applications. Whether you are conducting Western blotting, protein denaturation, or cell culture work, this reagent plays a critical role in maintaining protein integrity by preventing unwanted oxidation.

In modern research labs, maintaining sample quality is essential—and having standardized reagents ensures accuracy and reproducibility. Beta Mercaptoethanol, known for its strong reducing capabilities, continues to be a trusted tool across numerous scientific disciplines.

What Is Beta Mercaptoethanol (50 mM in PBS)?

• Beta Mercaptoethanol, also known as 2-mercaptoethanol, is a potent reducing agent widely used to break disulfide bonds in proteins. When formulated at 50 mM in PBS (Phosphate-Buffered Saline), it offers a stable, ready-to-use solution ideal for sensitive applications such as:
• Western Blot Reagents
• Protein sample preparation
• Enzyme preservation
• Cell culture supplementation
• General molecular biology workflows
• This formulation ensures consistency, minimizes preparation errors, and delivers optimal performance in experiments requiring reducing conditions.

Specifications of Beta Mercaptoethanol (50 mM in PBS)  

Cell Type: Mammalian Cells 
Concentration: 55 mM 
Quantity: 50 mL 
HS-Code: 29309095 
Sterile: Yes 
Form: Liquid 
Product Type: BME 
pH: 6 to 8 
Unit Size: Each 
Storage: 2°C to 8°C 
Shipping conditions: Room temperature 
Shelf life: 36 months from date of manufacture 

Key Features of Beta Mercaptoethanol (50 mM in PBS)

• Ready-to-use 50 mM concentration: Perfectly optimized for molecular biology and blotting workflows.
• High-purity beta mercaptoethanol: Ensures reliable reduction of disulfide bonds in proteins.
• Prepared in PBS buffer: Maintains physiological pH and salt balance for improved stability.
• Potent reducing agent: Prevents oxidation, maintains protein conformation, and preserves sample quality.
• Ideal for Western Blot Reagents & Blot Reagents: Supports clear, accurate, and reproducible protein separation and transfer.
• Consistent, lab-grade performance: Suited for research labs requiring dependable reagents for daily workflows. 

Benefits of Using Beta Mercaptoethanol (50 mM in PBS) 

• Enhances Protein Stability: By reducing disulfide bonds, it prevents protein aggregation and maintains structural integrity during experiments.
• Saves Preparation Time: A pre-made 50 mM solution eliminates the need to dilute hazardous stock, reducing handling risks.
• Improves Western Blot Results: Ensures proper protein denaturation for sharper bands and improved antibody binding.
• Reduces Experimental Errors: Standard formulation provides consistency across multiple assays and projects.
• Suitable for a Wide Range of Lab Applications: From molecular biology to enzyme studies, it is a versatile Lab Reagent trusted by scientists worldwide. 

Conclusion

Beta Mercaptoethanol (50 mM in PBS) is an essential Lab Chemical that offers reliability, consistency, and top-tier performance for protein-based and molecular biology applications. As a potent reducing agent, it protects protein structure, boosts assay accuracy, and streamlines workflows—making it a valuable addition to any research laboratory.

For scientists working with Western Blot Reagents, protein analysis, or molecular biology, this reagent remains a dependable choice for delivering high-quality, reproducible results.