Specifications of DuoSet ELISA Substrate Reagent Pack

16 vials of TMB ELISA Substrate (Part # 642736); 
12 mL/vial. 
Store unopened: at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C. 
Warm to room temperature before use.
Protect from light Add 100 μL to each well and incubate at room temperature for 20 minutes while.  
And Add Stop Solution (Catalog # DY994B or DY994B-250) and read the microplate.