Anti-Rabbit IgG HRP-linked Antibody: Reliable Detection for Immunoassays 

In the world of immunology and protein research, antibody-based detection systems are vital for achieving accurate and reproducible results. Among these tools, the Anti-Rabbit IgG HRP-linked Antibody stands out as a highly sensitive and specific reagent designed to support applications such as Western blotting, ELISA, immunohistochemistry, and other immunoassay techniques.

This antibody is engineered for researchers who need a robust secondary antibody that ensures clear, precise, and consistent results across experiments. 

What is an Anti-Rabbit IgG HRP-linked Antibody?

The Anti-Rabbit IgG antibody is a secondary antibody that binds specifically to rabbit immunoglobulin G (IgG) molecules. It is conjugated with Horseradish Peroxidase (HRP), an enzyme that catalyzes colorimetric reactions for easy detection of target antigens.

When paired with a suitable substrate such as TMB (3,3′,5,5′-Tetramethylbenzidine) or DAB (3,3′-Diaminobenzidine), this conjugate produces a strong, measurable signal, making it an essential component in diagnostic and research laboratories. 

Specifications of Anti-Rabbit IgG HRP-linked Antibody 

Applications: Western Blot
Sample: MIA PaCa-2 Human Pancreatic Tumor Cell Lysate
Primary Incubation: Cell Signaling GAPDH, 1:5000 dilution, 8 hours at 4 degrees Celsius in TBS-T
Blocking Agent: 5% Milk in TBS-T
Secondary Incubation: Cell Signaling Anti-Rabbit, HRP-linked secondary, 1:5000 dilution, 1 hour at room temperature in TBS-t
Tertiary Incubation: None  

Key Features of Anti-Rabbit IgG HRP-linked Antibody

• High Sensitivity: Detects even low-abundance proteins with excellent clarity.
• Superior Specificity: Binds specifically to rabbit IgG with minimal cross-reactivity to other species.
• Enzyme Conjugation: Linked with Horseradish Peroxidase (HRP) for enhanced colorimetric or chemiluminescent detection.
• Versatile Application: Ideal for Western blotting, ELISA, immunohistochemistry (IHC), and dot blot assays.
• Stable Formulation: Provides long-term stability when stored properly at 4°C.
• Ready-to-Use Solution: Available in convenient formats for immediate use in laboratory workflows.

Benefits of Using Anti-Rabbit IgG HRP-linked Antibody

• Accurate Detection: Ensures strong signal-to-noise ratio for improved data reliability.
• Time-Efficient: HRP conjugation allows for rapid color development and easy visualization.
• Cost-Effective: Delivers high performance at a competitive price, reducing assay optimization costs.
• Compatible with Multiple Assays: Works seamlessly across a wide range of immunological techniques.
• Consistent Results: Each batch is tested to guarantee reproducibility and quality control.

Applications

• Western Blotting (WB): Detects rabbit primary antibodies with clear band visualization.
• ELISA (Enzyme-Linked Immunosorbent Assay): Enables sensitive detection of antigen-antibody interactions.
• Immunohistochemistry (IHC): Used to localize antigens within tissue sections.
• Dot Blot and Immunoprecipitation: Provides strong signal detection with minimal background noise.

Storage and Stability 

To ensure maximum activity, store the Anti-Rabbit IgG HRP-linked Antibody at 4°C. Avoid repeated freeze-thaw cycles to maintain enzyme functionality and antibody integrity. 

Conclusion 

The Anti-Rabbit IgG HRP-linked Antibody is an indispensable tool for any research laboratory that requires reliable, high-quality detection of rabbit-derived primary antibodies. Its superior sensitivity, specificity, and HRP-based detection make it a top choice for scientists striving for accurate and reproducible immunoassay results.