DTT (Dithiothreitol) Reagent: A Critical Tool for Reliable Protein Research

In modern molecular biology and biochemistry laboratories, maintaining protein integrity is essential for generating accurate and reproducible results. DTT (Dithiothreitol) Reagent is one of the most widely used reducing agents, especially in workflows involving sensitive protein samples. As a dependable Lab Reagent, DTT plays a central role in protein stabilization, structural analysis, and preparation processes across a variety of applications.

Whether it’s used in Western Blot Reagents, enzyme studies, or routine protein assays, DTT offers exceptional performance due to its powerful reducing capabilities. Its ability to break disulfide bonds makes it a cornerstone reagent for scientists working with delicate protein samples.

What is DTT (Dithiothreitol) Reagent?

Dithiothreitol commonly known as DTT, is a strong reducing agent widely used in research laboratories. Due to its specific action on protein disulfide bridges, it is particularly valuable in sample preparation for Western blotting, protein purification, and molecular biology experiments. As one of the essential Lab Chemicals, DTT ensures accurate structural evaluation and prevents unwanted protein aggregation.

Specifications of DTT (Dithiothreitol) Reagent

Product Type: DTT (Dithiothreitol) 
Chemical Formula: C4H10O2S2 
CAS Number:  3483-12-3
Molecular Weight: 154.25 
Volume: 5 g 
Concentration: 0.1 M 
Unit Size: Each  
Package Information: Bottle 

Key Features of DTT (Dithiothreitol) Reagent 

• High-Purity Reducing Agent – Ideal for sensitive biochemical experiments requiring consistent performance.
• Efficient Disulfide Bond Reduction – Breaks intramolecular and intermolecular disulfide bonds in proteins.
• Stable and Reliable – Formulated for dependable use in various workflows involving protein preparation.
• Compatible with Western Blot Reagents – Ensures proper denaturation and accurate migration of proteins.
• Versatile Lab Reagent – Suitable for DNA/RNA preparation, enzyme protection, and structural studies.
• Long Shelf Stability – Designed to maintain effectiveness when properly stored.

Benefits of Using DTT (Dithiothreitol) Reagent  

• Improves Protein Sample Integrity: DTT prevents oxidation by maintaining proteins in their reduced form, ensuring more accurate downstream analysis.
• Enhances Western Blot Accuracy: As part of Blot Reagents, DTT ensures complete denaturation, allowing proteins to migrate correctly and produce clean, reliable bands.
• Increases Reproducibility: Using a high-quality reducing reagent minimizes experimental variability, which is critical for publishing and data comparison.
• Supports a Wide Range of Lab Applications: From molecular cloning to enzyme assays, DTT’s versatility makes it an essential component of many laboratory workflows.
• Reduces Protein Aggregation: By preventing disulfide-mediated interactions, DTT preserves sample quality and protects proteins from unwanted structural changes.

Conclusion

DTT (Dithiothreitol) Reagent 5 g is an indispensable tool for scientists who require precision, reliability, and consistency in protein research. Its powerful reducing capabilities make it crucial for applications involving Western Blot Reagents, protein denaturation, and general biochemical analysis. As a trusted Lab Chemical and essential Lab Reagent, DTT continues to play a vital role in ensuring high-quality laboratory results.

For laboratories committed to accuracy and performance, DTT remains a foundational reagent that supports dependable scientific discovery.